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Characteristics of the primers used to synthesize the probes
| Probe | PCR fingerprinting band detected (bp) | Forward primer | Reverse primer | Taa (°C) | Product size (bp) |
|---|---|---|---|---|---|
| P420 | 420 | GGTTGCCCAAACCCTCA | CCTTGGCATCAGCAACACAT | 58 | 870 |
| P475 | 475 | TTGATGTGAATCTTTTATACACTTGC | AGCGTGGAGCGTGGAG | 58 | 535 |
| P540 | 540 | GAACCCACCGCCCTCTTC | ACCAGACGGCAACCTTCTTA | 58 | 838 |
| P800 | 800 | ATCGCGTAGCATTTGAGCTT | ACAAATGTGGCGATGTGAGT | 58 | 900 |
Ta, annealing temperature.
