TABLE 3.
Alignment of primer sequences with contig matched in serotype A and serotype D genome databases
| Genome strain and band (bp) | Contig | Nucleotide identitya (%) for:
|
|||
|---|---|---|---|---|---|
| (GACA)4 primer
|
Multiplex PCR primers
|
||||
| Forward | Reverse | Forward | Reverse | ||
| H99 | |||||
| 420 | 1.84 | 10/16 (63) | 12/16 (75) | 15/17 (88) | 16/17 (94) |
| 475 | 1.5 | 12/16 (75) | 11/16 (69) | 18/18 (100) | 16/16 (100) |
| 540 | 1.35 | 11/16 (69) | 12/16 (75) | 16/17 (94) | 17/17 (100) |
| 800 | 1.32 | 12/16 (75) | 8/16 (50) | 17/17 (100) | 19/19 (100) |
| JEC21 | |||||
| 420 | C119 | 11/16 (69) | 13/16 (81) | 17/17 (100) | 17/17 (100) |
| 475 | C1135 | 12/16 (75) | 7/16 (44) | 11/18 (61) | 15/16 (94) |
| 540 | C592 | 12/16 (75) | 12/16 (75) | 17/17 (100) | 17/17 (100) |
| 800 | NMb | ||||
a
Number of identical sequences/total number of sequences.
b
NM, no match.