TABLE 4.
Nucleotide sequences at 5′ and 3′ ends of the four PCR fingerprinting major bands
| Strain (band [bp]) or primer | 5′ end
|
3′ end
|
Amplification resultc | ||
|---|---|---|---|---|---|
| Nucleotide sequencea | Nucleotide identity (%) | Nucleotide sequence | Nucleotide identity (%) | ||
| Primer | GACAGACAGACAGACA | TGTCTGTCTGTCTGTC | |||
| H99 (420) | ***AG*CA*A*AGACA | 63 | TGTCTG*C*GTC*GT* | 75 | − |
| JEC21 (420) | ***AG*CA*ACAGACA | 69 | TGTCTG*CTGTC*GT* | 81 | + |
| H99 (475) | *A**GACAGAC*GACA | 75 | TGTC*G*CTGTC**T* | 69 | + |
| JEC21 (475) | *A**GACAGAC*GACA | 75 | TGTC*G**T*****T* | 44 | − |
| H99 (540) | **C*G*CAGACAG*CA | 69 | TGTCTGT*TGTC*G** | 75 | + |
| JEC21 (540) | **CAG*CAGACAG*CA | 75 | TGTCTGT*TGTC***C | 75 | + |
| H99 (800) | *AC***CAGACAG*CA | 75 | TGT*TGTC****T*** | 50 | + |
| JEC21 (800) | NMb | NM | − | ||
a
*, nucleotide mismatch.
b
NM, no match.
c
−, no amplification; +, band amplification.