Unrooted neighbor-joining tree of 33 FCV consensus sequences (including the published sequence FCV F9 [GenBank accession number M86379]) for a 420-nucleotide region of the FCV capsid gene. Black squares, virus isolated from swab obtained at arrival in admissions; gray squares, virus isolated after arrival in admissions; black circles, virus isolated after arrival in main accommodation; black diamonds, virus isolated on arrival swab in isolation; gray diamonds, virus isolated after arrival in isolation. Each virus sequence is identified by the number of the cat from which it was obtained and the date (day.month.year) on which virus sample was taken. A, B, and C, the groups of cats involved in the transmission events within the shelter. Evolutionary distances were calculated by using the Jukes-Cantor model, with the scale bar indicating percentage divergence. Numbers at major nodes are the bootstrap values ≥80% of 1,000 replicates (MEGA program, version 3.0).