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. 2007 Jul 11;81(18):9838–9850. doi: 10.1128/JVI.00792-07

FIG. 1.

FIG. 1.

IDO induction by PIC, IFN-γ, and IFN-β in primary human astrocytes. (A) Astrocyte cultures were stimulated with 10 μg/ml of PIC or 10 ng/ml of recombinant IFN-γ or recombinant IFN-β for 1 or 3 days. The Western blot shows IDO (∼45 kDa) induction by all stimuli, with IFN-γ being the most potent and IFN-β the least potent. Blots were stripped and reprobed for vinculin (117 kDa) as a control for protein loading. (B) Dose-dependent IDO induction in astrocytes. Astrocyte cultures were stimulated with 10 U (0.125 ng/ml), 100 U, or 1,000 U of recombinant IFN-β, 10 U (0.5 ng/ml), 100 U, or 1,000 U of recombinant IFN-γ, or 0.1 μg/ml, 1 μg/ml, or 10 μg/ml of PIC for 1 day. Blots were probed for IDO and then stripped and reprobed for vinculin. Astrocyte IDO is induced in a dose-dependent manner with the following ranking of potency: IFN-γ > PIC > IFN-β. Results are representative of three independent experiments.