FIG. 3.

PIC-induced expression of IDO is dependent on NF-κB and IRF-3. (A) Astrocytes were infected for 48 h with increasing concentrations (multiplicities of infection) of adenoviral vectors expressing a DN NF-κB (srIκBα) or EV. Cells were then stimulated with 10 μg/ml of PIC for 24 h, and total cell homogenates were prepared and immunoblotted for IDO and vinculin. PIC-induced IDO was dose dependently inhibited by the SR IκBα but not EV. Adenovirus expressing the SR IκBα or EV alone did not induce IDO. (B) The role of DN IRF3 was examined by adenoviral vectors as for panel A. The results showed that IDO expression was dose dependently inhibited by DN IRF3, whereas EV were without effect. The data shown are from one of two separate experiments with identical results.