FIG. 5.

Immunoblot analysis of mutant HIV-1 particles encoding amino acid substitutions in the gp41 CT. Virus lysates (A) and cell lysates (B) were normalized by p24 ELISA and total protein levels, respectively, and subjected to SDS-PAGE and immunoblotting with the indicated antibodies. (C) Relative Env expression was determined as the ratio of gp41 to Nef in cell lysates, and Env incorporation into particles was determined as the ratio of gp41 to CA in viral lysates. (D) The gp120/gp41 ratios were calculated and expressed as a percentage of the wild-type value. Data shown in panels C and D are the mean values and standard deviations obtained from three independent experiments.