TABLE 2.

Primers used for construction of alanine-scanning CT mutants

Mutation	Primer sequencea
VNL812AAA	(S) 5′-GAAAGGGCTGCAGCCTAAGATGGGTGGCAAGTGG
	(A) 5′-ATCTTAGGCTGCAGCCCTTTCCAAGCCCTGTCTTATTC
LN814AA	(S) 5′-CAGGGCGCTGCAGCGATTTTGCTATAAGATGGGTGG
	(A) 5′-GCAAAATCGCTGCAGCGCCCTGTCTTATTCTTCTAGG
TAI818AAA	(S) 5′-AGAATAGCTGCAGCCTTGGAAAGGATTTTGCTATAAG
	(A) 5′-TTCCAAGGCTGCAGCTATTCTTCTAGGTATGTGGCG
V820A	(S) 5′-ATACCTGCTGCAGCCAGACAGGGCTTGGAAAGGATT
	(A) 5′-CTGTCTGGCTGCAGCAGGTATGTGGCGAATAGCTC
EGT824AAA	(S) 5′-ATTCGCGCTGCAGCCAGAAGAATAAGACAGGGCTTGG
	(A) 5′-TCTTCTGGCTGCAGCGCGAATAGCTCTATAAGCTGC
DRV827AAA	(S) 5′-TATAGAGCTGCAGCCCACATACCTAGAAGAATAAGAC
	(A) 5′-ATGTGGGCTGCAGCTCTATAAGCTGCTTGTAATAC
IEV830AAA	(S) 5′-CAAGCAGCTGCAGCCGCTATTCGCCACATACCTAG
	(A) 5′-AATAGCGGCTGCAGCTGCTTGTAATACTTCTATAACC
LQ832AA	(S) 5′-GAAGTAGCTGCAGCGGCTTATAGAGCTATTCGCC
	(A) 5′-ATAAGCCGCTGCAGCACTTCTATAACCCTATCTGTCC
YR836AA	(S) 5′-AGGGTTGCTGCAGCCTTACAAGCAGCTTATAGAGC
	(A) 5′-TTGTAAGGCTGCAGCAACCCTATCTGTCCCCTCAGC
IR839AA	(S) 5′-GGGACAGCTGCAGCGATAGAAGTATTACAAGCAGC
	(A) 5′-TCTATCGCTGCAGCTGTCCCCTCAGCTACTGCTATGG
HIP842AAA	(S) 5′-GTAGCTGCTGCAGCCGATAGGGTTATAGAAGTATTAC
	(A) 5′-CCTATCGGCTGCAGCAGCTACTGCTATGGCTGTGGC
RRI845AAA	(S) 5′-GCCATAGCTGCAGCCGAGGGGACAGATAGGGTTATAG
	(A) 5′-CCCCTCGGCTGCAGCTATGGCTGTGGCATTGAGC
RQG848AAA	(S) 5′-AATGCCGCTGCAGCGGCAGTAGCTGAGGGGACAG
	(A) 5′-GGCTGTGGCATTGAGCAAGTTAACAGCACTATTCTT
LER851AAA	(S) 5′-AACTTGGCTGCAGCCACAGCCATAGCAGTAGCTGAGG
	(A) 5′-GGCTGTGGCATTGAGCAAGTTAACAGCACTATTCTT
ILL854AAA	(S) 5′-AGTGCTGCTGCAGCACTCAATGCCACAGCCATAGC
	(A) 5′-ATTGAGTGCTGCAGCAGCACTATTCTTTAGTTCC

^aS, sense primer; A, antisense primer.