TABLE 3.

Recognition of altered peptide ligands: subject 2^a

Peptide		% Specific lysis by clone
Variant	Sequence	2H1	9C8	7E6	1C12	4B4	9C12	3G5	2B10	8A10	9A2	9E1	1C10	3H12	6G8	8G3	8H2
D1 KP9	KPWDVIPMV	100	100	100	100	100	100	100	100	100	100	100	100	100	100	100	100
D2-1	KPWDIIPMV	91	0	101	100	181	101	94	101	14	97	94	84	87	57	26	76
D2-2	KPWDVVPMV	52	132	124	121	229	122	95	99	127	122	94	84	95	97	84	91
D2-3	KPWDVLPMV	100	102	85	8	184	120	87	89	96	3	11	93	62	97	94	10
D2-4	KPWDVLPTV	94	56	124	22	17	120	70	8	118	0	6	20	54	77	9	6
D3	KPWDVVPTV	24	132	114	99	178	122	106	75	109	77	66	25	118	103	28	53

^aAltered peptide ligands representing heterologous dengue viruses are differentially recognized by Den-1 NS5_329-337-specific CTL clones. Bolded letters indicate the variant amino acid(s). CTL clones generated by specific stimulation with the cognate peptide KP9 were tested against a panel of altered peptide ligands representing heterologous serotypes in a 5-h ⁵¹Cr release assay. Peptides were included at a final concentration of 5 μg/ml. Results are expressed as a percentage of specific lysis induced by cognate D1 KP9 epitope peptide.