TABLE 6.
Den-1 NS5329-337-specific CTL clones differentially secrete Tc1 and Tc2 cytokines on stimulation with cognate Den-1 KP9 peptide and altered peptide ligands representing heterologous dengue virusesa
| Subject and clone | Peptide | Cytolysis (%)b | Response (pg/ml culture supernatant)
|
|||||
|---|---|---|---|---|---|---|---|---|
| IFN-γ | TNF-α | IL-2 | IL-4 | IL-6 | ||||
| Subject 2 | ||||||||
| 1H2 | KP9 | 100 | >2,000 | 252 | 8 | 10 | −c | |
| D2-1 | 89 | 1,964 | 120 | 10 | 10 | − | ||
| D2-2 | 84 | 838 | 43 | 22 | − | − | ||
| D2-3 | 85 | 2,241 | 157 | 11 | 6 | − | ||
| D2-4 | 94 | 1,177 | 115 | 8 | − | − | ||
| D3 | 14 | 66 | − | − | − | − | ||
| 6C11 | KP9 | 100 | >2,000 | 109 | − | − | − | |
| D2-1 | 64 | 237 | 9 | − | − | − | ||
| D2-2 | 91 | 626 | 22 | − | − | − | ||
| D2-3 | 82 | 1,233 | 36 | − | − | − | ||
| D2-4 | 50 | 155 | 8 | − | − | − | ||
| D3 | 3 | 63 | − | − | − | − | ||
| 1D4 | KP9 | 100 | >2,000 | 1,498 | 197 | 171 | − | |
| D2-1 | 58 | 99 | 11 | 89 | − | − | ||
| D2-2 | 98 | >2,000 | 1,239 | 298 | 126 | − | ||
| D2-3 | 115 | >2,000 | 1,143 | 197 | 129 | − | ||
| D2-4 | 100 | >2,000 | 1,913 | 298 | 182 | − | ||
| D3 | 94 | >2,000 | 1,732 | 325 | 150 | − | ||
| 1B4 | KP9 | 100 | >2,000 | 139 | 43 | 8 | − | |
| D2-1 | 37 | 111 | 15 | 79 | − | − | ||
| D2-2 | 24 | 31 | − | 79 | − | − | ||
| D2-3 | 93 | >2,000 | 212 | 141 | 8 | − | ||
| D2-4 | 87 | 1,182 | 90 | 119 | − | − | ||
| D3 | 5 | 21 | − | 43 | − | − | ||
| 10G12 | KP9 | 100 | >2,000 | 153 | 87 | − | − | |
| D2-1 | 35 | 236 | 33 | 114 | − | − | ||
| D2-2 | 25 | 25 | − | 103 | − | − | ||
| D2-3 | 97 | 1,800 | 156 | 98 | − | − | ||
| D2-4 | 96 | 1,205 | 84 | 109 | − | − | ||
| D3 | 8 | − | − | 120 | − | − | ||
| 2C9 | KP9 | 100 | >2,000 | 202 | − | 8 | − | |
| D2-1 | 27 | 220 | 30 | − | − | − | ||
| D2-2 | 12 | 70 | 7 | − | − | − | ||
| D2-3 | 97 | >2,000 | 224 | 19 | 14 | − | ||
| D2-4 | 91 | 1,582 | 124 | 12 | − | − | ||
| D3 | 3 | 21 | − | − | − | − | ||
| Subject 3 | ||||||||
| 7E6 | KP9 | 100 | 188 | − | − | − | − | |
| D2-1 | 101 | 156 | − | − | − | − | ||
| D2-2 | 124 | 120 | 45 | − | − | − | ||
| D2-3 | 85 | 104 | − | − | − | − | ||
| D2-4 | 124 | 90 | − | − | − | − | ||
| D3 | 114 | 108 | − | − | − | − | ||
| 1C12 | KP9 | 100 | >2,000 | − | 164 | − | − | |
| D2-1 | 100 | >2,000 | − | 154 | − | − | ||
| D2-2 | 121 | >2,000 | − | 91 | − | − | ||
| D2-3 | 8 | − | − | − | − | |||
| D2-4 | 22 | − | − | − | − | |||
| D3 | 99 | 1,535 | − | − | − | − | ||
| 4B4 | KP9 | 100 | 1,668 | − | − | − | − | |
| D2-2 | 229 | >2,000 | − | − | 479 | − | ||
| D2-1 | 181 | >2,000 | − | − | − | − | ||
| D2-3 | 184 | 953 | − | − | − | |||
| D2-4 | 17 | 107 | − | − | 44 | − | ||
| D3 | 178 | 452 | − | − | − | − | ||
| 9C12 | KP9 | 100 | >2,000 | − | − | − | − | |
| D2-1 | 101 | >2,000 | − | − | − | − | ||
| D2-2 | 122 | >2,000 | − | − | − | − | ||
| D2-3 | 120 | 1,440 | − | − | − | − | ||
| D2-4 | 120 | 813 | − | − | − | − | ||
| D3 | 122 | 1,730 | − | − | − | − | ||
| 2B10 | KP9 | 100 | >2,000 | 206 | 225 | 34 | 196 | |
| D2-1 | 101 | >2,000 | 138 | 413 | 38 | 286 | ||
| D2-2 | 99 | >2,000 | 1,653 | − | 363 | − | ||
| D2-3 | 89 | >2,000 | − | 54 | − | 55 | ||
| D2-4 | 8 | 137 | − | − | − | − | ||
| D3 | 75 | 1,516 | − | 39 | − | − | ||
| 1C10 | KP9 | 100 | >2,000 | 1,870 | 239 | 189 | 70 | |
| D2-1 | 84 | >2,000 | 1,154 | 77 | 117 | 67 | ||
| D2-2 | 84 | >2,000 | 800 | 35 | 85 | 64 | ||
| D2-3 | 93 | >2,000 | 980 | 93 | 106 | 58 | ||
| D2-4 | 20 | >2,000 | 51 | − | − | 62 | ||
| D3 | 25 | >2,000 | 44 | − | − | 49 | ||
a
Peptides were added at a final concentration of 5 μg/ml. Supernatants were also tested for IL-10; results for IL-10 were always below the limit of detection.
b
Cytolysis is reported as a percentage of the cognate KP9 peptide epitope lysis value, corrected to 100%.
c
−, below detectable limits of the assay.