FIG. 3.

Effect of deletion of either Gn or Gc CT on BUNV glycoprotein maturation. Vero cells were infected with vTF7-3 and subsequently transfected with wt or mutant BUNV M segment cDNA constructs. Cells were labeled with [³⁵S]methionine for 20 min and chased in the presence of excess unlabeled methionine for different times, as indicated (in minutes). Labeled proteins were immunoprecipitated (IP) with either MAb 742 (A) or anti-BUNV (B), subjected to endo H digestion (+) or left undigested (−) as indicated, and analyzed by SDS-12.5% PAGE under reducing conditions. BUNV Gn and Gc proteins are indicated to the right of the gels. Quantitative analyses of protein bands by phosphorimaging are shown. Data from the pulse-chase experiment with MAb 742 are shown as the total density at each time point, while data from the pulse-chase experiment with anti-BUNV are shown as percentages of the endo H-resistant form versus the total Gc protein.