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Effects of mutations in CT domains of Gn and Gc on the formation of infectious VLPs. BSR-T7/5 cells were transfected with minigenome component plasmids together with either wt BUNV M segment cDNA or alanine-substituted mutants. Supernatants from these cells were taken at 24 h posttransfection and used to infect fresh BSR-T7/5 cells. Renilla luciferase activities in extracts of these cells were measured after another 24 h of incubation and are shown in arbitrary light units.
