FIG. 7.

C-terminal mutations of ORF6 affect STAT1 localization. (A) Schematic of ORF6 alanine mutants expressed in B to D. Numbers correspond to amino acids in ORF6. The black box indicates the amino acids that are changed to alanine in the protein (B) Each alanine mutant was fused to a C-terminal GFP and expressed in Vero cells. Localization is shown via a GFP filter on a confocal microscope. (C) Each HA-tagged alanine mutant was cotransfected with STAT1/GFP. At 24 h posttransfection, cells were treated with 100 IU/ml of IFN-β for 60 min before fixation in 4% PFA. STAT1/GFP was visualized on a confocal microscope.