FIG. 3.

CTAR1 induces activation of PI3K-Akt signaling and deregulates markers associated with cell cycle progression. (A) Rat-1 cells stably expressing pBabe-puromycin (pBabe), LMP1, or LMP1 mutants were assayed by Western blot analysis for expression of LMP1 constructs with antibodies against HA, phosphorylated GSK3β (p-GSK3β) (S9), total GSK3 (α and β forms), plakoglobin, CDK2 (hyperphosphorylated CDK2 is marked by the arrowhead), total Rb, and actin as a loading control. (B) Quantitative analysis of protein levels of pGSK3b (white bars), plakoglobin (black bars), and Rb (stippled bars). Values are changes in induction compared to the level induced by pBabe-puromycin (pBabe).