TABLE 1.
Summary of activities of LMP1 and LMP1 mutantsa
| Construct | Transforming ability | Inhibition by U0126 | Level of proteinb
|
|||
|---|---|---|---|---|---|---|
| p-GSK3β | p27Kip1 | Plakoglobin | p-ERK1/2 | |||
| LMP1 | Yes | Yes | ++++ | −−−− | −−− | ++++ |
| LMP1-A5 | No | Yes | +++ | −−−− | −−−− | NC |
| LMP1-Δ204-208 | No | Yes | ++ | −−−− | −−−− | NC |
| LMP1-204/6AA | Yesc | Yes | ++ | −−− | −−− | +++ |
| LMP1-208A | Yes | Yes | +++ | −− | −−− | ++++ |
| LMP1-Y384G | Yes | Yes | ++ | −−− | −−− | ++++ |
| A5-Y384G | No | Yes | +++ | −−−− | −−−− | NC |
| LMP1-378Stop | Yes | Yes | ++ | −−− | −−− | ++++ |
| A5-378Stop | No | Yes | +++ | −−− | −−−− | NC |
| 1-187 | No | Yes | + | ++ | − | NC |
| 1-195 | No | Yes | + | ++ | − | NC |
| 1-203 | No | Yes | + | +++ | NC | NC |
| 1-208 | No | Yes | +++ | −−−− | −−−− | NC |
| 1-208-A5 | No | Yes | + | +++ | + | NC |
| 1-220 | Yes | Yes | ++++ | −−−− | −−−− | ++++ |
| 1-220-A5 | No | Yes | + | +++ | + | NC |
| 1-231 | Yes | Yes | ++++ | −−−− | −− | ++++ |
a
LMP1 and LMP1 mutants that are transforming are shown in boldface type.
b
The level of protein (phosphorylated GSK3β [p-GSK3β] and phosphorylated ERK1/2 [pERK1/2]) compared to the level of the control is shown. Plus signs indicate a level higher than that of the control, while minus signs indicate a level lower than that of the control. The number of symbols indicates levels of change from vector control, with more symbols indicating a greater level of change. NC, no change compared to that of the control.
c
Decreased transformation potential.