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. 1998 May 12;95(10):5768–5772. doi: 10.1073/pnas.95.10.5768

Figure 2.

Figure 2

Double-immunofluorescent staining of Lewis rat spinal cord for calpain (green) and cell-specific markers (red) (×200). (A) Calpain and GFAP (astrocytes) were colocalized (yellow) in normal spinal cord white matter. (B) Markedly increased calpain expression in reactive astrocytes of EAE (arrow). (C) Microglia (red) in normal spinal cord white matter. (D) Spinal cords from animals with EAE showed markedly increased calpain expression in reactive microglia (arrow). (E) Normal spinal cords stained for calpain and galactocerebroside (oligodendrocytes). (F) Oligodendrocytes in EAE showed a small increase in calpain immunofluorescence. (G) Calpain and CD2 (T cells) antibodies in controls often detected no T cells. (H) Spinal cords from EAE animals contained many T cells with limited calpain expression. (I) Normal spinal cords labeled with calpain and IFN-γ antibodies. (J) In EAE, calpain and IFN-γ expression were colocalized as both proteins appear inside and outside the activated T cells (arrow). (K) Macrophage (red) in normal spinal cord white matter. (L) Spinal cords of rats with EAE showed calpain expression in an activated macrophage (arrow).