FIG. 3.
PrPSc levels in brains and spleens of scrapie-infected and coinduced mice. Brain and spleen extracts from individual mice suffering from coinduced disease, scrapie-infected sick mice, or asymptomatic mice incubating scrapie for the designated times were digested with PK and immunoblotted with anti-PrP MAb IPC1. Each lane represents a 40-μl aliquot of a 10% brain homogenate or a 100-μl aliquot of a 10% spleen homogenate. For spleen samples, anti-mouse Fcγ fragment was used as a secondary antibody. The number under each lane designates the time of sacrifice for sick or asymptomatic mice. Asterisks represent individual terminally sick mice (coinduced or scrapie-infected mice), while the others were sacrificed in an asymptomatic state. i.c. (h), high-titer i.c. experiment; i.p., high-titer i.p. experiment; i.c. (l), low-titer i.c. experiment. dpi, days postinfection.