TABLE 1.
Quantitative evaluation of inflammation in coinduced mouse spinal cordsb
| Experimental group | Inoculum dilutiona | Demyelination (%) | No. of C3-stained cells/mm3
|
No. of MAC-stained cells/mm3 | Time of sacrifice (days postinfection)
|
||
|---|---|---|---|---|---|---|---|
| White matter | Gray matter | Scrapie | EAE | ||||
| EAE | 10−2 (NB) | 5 ± 4.7 | 80.5 ± 25.5 | 6 ± 3 | 42 ± 13 | 66 | |
| 10−8 (NB) | 2.5 ± 1.6 | 26.7 ± 4.4 | 11.7 ± 4.4 | 12.5 ± 1.3 | >170 | ||
| Scrapie | 10−2 (SB) | 0 | 4 ± 1 | 5 ± 2 | 0 | 96 | |
| 10−8 (SB) | 0 | 2.7 ± 1.7 | 2.3 ± 1.45 | 0 | >200 | ||
| Scrapie plus EAE | 10−2 (SB) | 19.06 ± 0.09* | 137 ± 36 | 29.5 ± 2.5* | 141.5 ± 45.5* | 96 | 66 |
| 10−8 (SB) | 6.6 ± 2.96 | 42.7 ± 14 | 25.3 ± 7.7 | 29 ± 9.86 | >200 | >170 | |
a
NB, normal brain homogenate; SB, scrapie-infected brain homogenate.
b
Data are means ± standard deviations. *, P < 0.05. Statistical evaluation was done using the t test and one-way analysis of variance to compare treatment groups.