FIG. 1.

KSHV replicons. (A) Schematic of plasmid constructs used in retention assays. GFP is inserted into pCRII (Invitrogen) at XhoI to XbaI; TRs or derivatives are at NotI; LANA is at EcoRV. Plasmid p2TRΔRE-GFP was derived from pTRΔ512-556 (described in reference 29) and contains two copies of TR in tandem, each lacking the G+C-rich region encompassing RE directly adjacent to LBS1/2. pRELBS1/2-GFP contains 71 bp of the minimal replicator inserted HindIII to NotI, while p3XLBS1/2-GFP contains three sets of minimal LBS1/2 at NotI in tandem. (B) LANA expression at 24 h in 293 cells transfected with 1 μg pLANA-2TR-GFP (lane 1) or p2TR-GFP control (lane 2). α-Tubulin was used as a loading control.