FIG. 3.

HPLC analysis of mutants. The complete HPLC chromatogram for wild-type NL4-3 virions is shown at the top of the figure with the region that is presented for the mutants indicated by arrows. The identities of the Gag proteins, determined by Coomassie brilliant blue-stained sodium dodecyl sulfate-polyacrylamide gel electrophoresis, immunoblotting, protein sequencing, and mass spectrometry, are identified above each respective peak. The mutant proteins as identified by MALDI-TOF mass spectrometry are shaded in black. The p6^Gag-containing species that are missing the C-terminal 16 amino acids due to cleavage after residue 36 are indicated by a “-cf” notation, e.g., p6^-cf, in the identifying superscript. The C-terminal fragment itself was labeled p6^cf when observed.