TABLE 1.
Summary of results of infection of sheep with wild-type and mutant BLV proviruses
| Sheepa | Type of virus | Provirus strain | Date (mo-yr) of:
|
Cause of deathi | Period (mos) of latencyj | Mean duration (mos) of latencyj | |
|---|---|---|---|---|---|---|---|
| Seroconversion | Death | ||||||
| 210 | Wild type | 344b | 02-91 | 01-94 | Leukemia | 35 | 36 |
| 214 | Wild type | 344 | 10-91 | 01-93 | Leukemia | 16 | |
| 224 | Wild type | 344 | 09-91 | 03-94 | Leukemia | 31 | |
| 230 | Wild type | 344 | 07-91 | 09-92 | Leukemia | 15 | |
| 235 | Wild type | 344 | 12-91 | 10-97 | Leukemia | 71 | |
| 236 | Wild type | 344 | 11-91 | 09-95 | Leukemia | 47 | |
| 238 | Wild type | 344 (Denv + Dpol)c | 05-92 | 02-96 | Leukemia | 46 | |
| 241 | Wild type | 344 (Denv + Dpol) | 04-92 | 07-95 | Leukemia | 40 | |
| 244 | Wild type | 344 | 05-92 | 12-95 | Leukemia | 44 | |
| 252 | Wild type | 344 | 01-93 | 07-95 | Leukemia | 31 | |
| 260 | Wild type | 344 (IX)d | 10-92 | 12-95 | Leukemia | 39 | |
| 261 | Wild type | 344 (IX) | 10-92 | 04-96 | Leukemia | 43 | |
| 273 | Wild type | 344 | 11-93 | 09-95 | Leukemia | 23 | |
| 274 | Wild type | 344 | 11-93 | 10-95 | Leukemia | 24 | |
| 286 | Wild type | 344 | 05-95 | 06-96 | Leukemia | 13 | |
| 293 | Wild type | 344 (IX) | 07-96 | 04-01 | Leukemia | 58 | |
| 8 | Wild type | 344 (IX) | 06-95 | 01-02 | Leukemia | 80 | |
| 11 | Wild type | 344 (IX) | 06-95 | 11-96 | Leukemia | 18 | |
| 31 | Wild type | 344 (IX) | 07-95 | 11-96 | Leukemia | 17 | |
| 298 | Wild type | 344 (IX) | 10-95 | 06-99 | Leukemia | 45 | |
| 4229 | Wild type | 344 | 10-04 | 04-06 | Leukemia | 19 | |
| 109 | Wild type | 344 (IX) | 12-97 | 06-99 | Unrelated | (19) | (23) |
| 110 | Wild type | 344 (IX) | 09-98 | 06-99 | Unrelated | (10) | |
| 2672 | Wild type | 344 (IX) | 10-99 | 10-03 | Unrelated | (49) | |
| 1095 | Wild type | 344 (IX) | 09-00 | 09-03 | Unrelated | (37) | |
| 292 | Wild type | 344 (IX) | 06-96 | 08-99 | Unrelated | (27) | |
| 4536 | Wild type | 344 | 02-02 | 05-03 | Unrelated | (16) | |
| 2158 | Wild type | 344 | 10-03 | 05-05 | Unrelated | (20) | |
| 4188 | Wild type | 344 | 10-04 | 04-05 | Unrelated | (7) | |
| 237 | G4 mutant | IG4e | 04-93 | 08-99 | Unrelated | (77) | (48) |
| 240 | G4 mutant | IG4 | 06-93 | 08-99 | Unrelated | (75) | |
| 249 | G4 mutant | IG4 | 05-93 | 01-95 | Unrelated | (21) | |
| 271 | G4 mutant | IG4 | 11-93 | 04-95 | Unrelated | (18) | |
| 272 | G4 mutant | IG4 | 11-93 | 10-00 | Unrelated | (84) | |
| 1071 | G4 mutant | IG4 | 10-00 | 07-04 | Unrelated | (46) | |
| 1077 | G4 mutant | IG4 | 10-00 | 09-04 | Unrelated | (48) | |
| 4537 | G4 mutant | IG4 | 02-02 | 03-04 | Unrelated | (26) | |
| 4538 | G4 mutant | IG4 | 02-02 | 12-04 | Unrelated | (35) | |
| 245 | G4-R3 mutant | DXf | 11-93 | 04-01 | Leukemia | 90 | 90 |
| 246 | G4-R3 mutant | DX | 05-92 | 03-00 | Unrelated | (95) | (71) |
| 262 | G4-R3 mutant | DX | 11-93 | 07-96 | Unrelated | (33) | |
| 264 | G4-R3 mutant | DX | 11-92 | 09-02 | Unrelated | (119) | |
| 278 | G4-R3 mutant | DX | 05-95 | 06-99 | Unrelated | (50) | |
| 279 | G4-R3 mutant | DX | 05-95 | 06-99 | Unrelated | (50) | |
| 1086 | G4-R3 mutant | DX | 09-00 | 02-04 | Unrelated | (42) | |
| 1091 | G4-R3 mutant | DX | 09-00 | 02-04 | Unrelated | (42) | |
| 255 | G4-R3 mutant with RZ | DX (RZ)g | 07-92 | 06-99 | Unrelated | (84) | |
| 256 | G4-R3 mutant with RZ | DX (RZ) | 10-92 | 05-96 | Unrelated | (44) | |
| 257 | G4-R3 mutant with RZ | DX (RZ) | 07-92 | 01-03 | Unrelated | (151) | |
| 277 | TM mutant | 6073 Tyrh | 07-94 | 05-01 | Leukemia | 83 | 83 |
| 276 | TM mutant | 6073 Tyr | 06-94 | 10-95 | Unrelated | (17) | (39) |
| 284 | TM mutant | 6073 Tyr | 05-95 | 06-99 | Unrelated | (50) | |
| 285 | TM mutant | 6073 Tyr | 05-95 | 06-99 | Unrelated | (50) | |
Data for animals 245 and 277 are highlighted in bold.
Provirus 344 was cloned from lymphomatotic BLV-infected sheep 344.
Denv and Dpol are mutants with deletions of env and pol, respectively. Cotransfection with these mutants is equivalent to transfection with the wild-type provirus.
The IX strain contains a deletion in untranslated sequences (viral RNA coordinates 6614 to 6731) but behaves as the wild type.
The IG4 mutant contains a stop codon in the G4 open reading frame at position 6697.
The DX mutant has a deletion between nucleotides 6614 and 6997 which affects both R3 and G4 genes.
DX (RZ) is identical to DX but contains a Tax-targeted ribozyme sequence (RZ) inserted in place of the 6614-6997 deletion.
The transmembrane protein (TM) mutant has a single substitution of G instead of T at position 6073, creating a tyrosine-to-aspartic acid change in the gp30 protein.
Causes including leukemia, lymphoma, and lymphosarcoma are indicated as leukemia. For those animals surviving to the end of the experiment or dying from accidental disease (mainly diarrhea and scabies) or other causes, the cause is listed as unrelated.
Number of months separating seroconversion and death due to leukemia, lymphoma, and/or lymphosarcoma. When the numbers are in parentheses, the death was due to unrelated causes.