FIG. 3.

Nuclear and nucleolar localization of wt KSHV ORF57 and mutants by immunofluorescence microscopy. HeLa cells were transfected with each mutant ORF57 or wt ORF57 plasmid and fixed 36 h after transfection. Slides were stained with anti-ORF57 specific antibody. The first panel (wt A) shows the appearance of the majority of wt ORF57 transfected cells, with fainter staining of the nucleoli than the surrounding nucleoplasm. The second panel shows the appearance of a minority of the cells in which nucleolar concentration was observed (wt B). The last two panels on the right show the ΔNLS mutant double stained with ORF57 antibody (ORF57) or anti-fibrillarin antibody to identify nucleoli (nucl).