FIG. 2.

Mapping of the TURBS with 3-nucleotide deletions. The sequence on top represents regions of the 3′-terminal end of ORF1, with the two essential regions determined by the 9-nucleotide deletions written in boldface. The numbers indicate the locations of the sequences with respect to the ORF1 TGA. Below, the positions of 3-nucleotide deletions in the first (left) and second (right) motifs are given. The gels show the proteins precipitated after transient expression, with the calculated VP2 expression efficiencies indicated below the autoradiographs (relative to wt pRmRNA, normalized to the expression level of VP1).