TABLE 6.

Viral nucleic acids in PBMC and cellular subsets^a

Cell subset	HIV-1 nucleic acid	No. of HIV nucleic acid copies/106 cells from:				No. of HIV nucleic acid-positive cells/106cells fromb:
		Untreated patients		Treated patients		Untreated patients		Treated patients
		Median	Min-max	Median	Min-max	Median	Min-max	Median	Min-max
Total PBMC	DNA	696	491-1,431	123	15-997
	usRNA	2,029	160-13,082	56	3.7-301	206	17-990	18	3-48
	msRNA	598	106-2,679	0.3	<0.1-9	56	7-125	0.4	<0.1-4
	vRNAex	33	3-201	<0.1	<0.1-0.5	10	3-46	<0.1	<0.1-3
Total CD4+	DNA	4,823	2,232-10,379	727	123-2,869
T cells	usRNA	4,020	379-21,554	342	44-1,410	473	64-9,278	111	8-247
	msRNA	1,259	85-5,158	4.1	<0.1-121	144	13-655	2	0.5-23
	vRNAex	1.3	<0.1-108	0.2	<0.1-19	3	<0.1-36	0.2	<0.1-1
Resting CD4+	DNA	2,198	253-8,962	585	146-1,898
T cells	usRNA	6,007	2,206-41,858	8.3	5-488	428	161-6,720	34	8-352
	msRNA	1,319	471-3,814	8.3	<0.1-118	96	39-269	8	<0.1-41
	vRNAex	0.5	<0.1-54	<0.1	<0.1-<0.1	1	<0.1-24	<0.1	<0.1-<0.1
Activated CD4+	DNA	6,388	2,240-29,054	1,248	690-2,989
T cells	usRNA	9,809	2,198-29,769	434	249-2,618	1,402	315-34,771	102	42-5,172
	msRNA	2,576	401-10,239	16	<0.1-59	256	33-590	3	<0.1-46
	vRNAex	2.3	<0.1-4	<0.1	<0.1-<0.1	15	<0.1-26	<0.1	<0.1-<0.1
CD4−/CD8−	DNA	166	55-541	<0.1	<0.1-47
T cells	usRNA	14,782	6,049-59,411	<0.1	<0.1-6	427	14-1,485	<0.1	<0.1-6
	msRNA	7,775	370-14,617	<0.1	<0.1-<0.1	474	2-1,485	<0.1	<0.1-<0.1
	vRNAex	192	23-1,737	<0.1	<0.1-0.2	101	9-282	<0.1	<0.1-0.2
Monocytes	DNA	<0.1	<0.1-8	<0.1	<0.1-5
	usRNA	21	0.7-233	<0.1	<0.1-3	5	0.5-35	0.3	<0.1-0.7
	msRNA	<0.1	<0.1-191	<0.1	<0.1-0.6	<0.1	<0.1-7	0.2	<0.1-0.7
	vRNAex	1	<0.1-8	<0.1	<0.1-0.3	0.7	<0.1-3	<0.1	<0.1-0.4

^aIf no HIV RNA was detected, the limit of detection (0.1 copy per 10⁶ cells or 0.1 positive cell per 10⁶ cells of each subset) was used for statistical analyses. Min-max, minimum to maximum.

^bHIV DNA was not assessed by limiting dilution analysis.