FIG. 3.

Replication kinetics of viable cleavage mutants and MHV9/10 revertants. (A) Comparison of growth curves for MHV (black circle and solid line), MHV7/8A (black square and solid line), MHV7/8B (black triangle and dashed line), and MHV9/10 (black circle and dashed line). (B) Replication fitness of revertant MHV9/10 passage 15 viruses. Growth curves compare MHV (black circle and solid line) to the passage 15 isolates MHVp15-1 (black square and solid line) and MHVp15-3 (black triangle and dashed line). Growth curves were performed on DBT cell monolayers infected at an MOI of 0.05 PFU/cell. Supernatants were sampled for replicating virus at 0, 6, 12, 18, and 24 hpi, and titers were determined by plaque assay. Data points represent the average of three replicate experiments, and error bars show the standard deviation. The limit of detection (lod) is represented by a horizontal dashed line at 1.7 log₁₀ PFU/ml.