TABLE 3.
Mutagenesis of the nsp7 to nsp10 cleavage sites
| Virusa | Mutation of P1 (Q to A) at cleavage sitesb
|
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| P5 | P4 | P3 | P2 | P1 | P1′ | P2′ | P3′ | P4′ | P5′ | |
| MHV6/7 | V | S | Q | I | A | S | R | L | T | D |
| MHV7/8A* | L | A | A | L | Q | S | E | F | V | N |
| MHV7/B† | L | Q | A | L | A | S | E | F | V | N |
| MHV7/8AB‡ | L | A | A | L | A | S | E | F | V | N |
| MHV8/9 | T | V | V | L | A | N | N | E | L | M |
| MHV9/10 | T | V | R | L | A | A | G | T | A | T |
| MHV10/11 | G | S | Q | F | A | S | K | D | T | N |
a
Symbols: *, the upstream LQ↓A site is substituted; †, the downstream LQ↓S site is substituted; ‡, both sites are substituted.
b
Boldface type, introduced Ala.