FIG. 5.

Sulfated glycans have no detectable effect on the uptake of abnormal PrP by Rov cells. Immunoblots of abnormal PrP taken up by Rov cells are shown. (A) Duplicate wells were left untreated or were treated for 24 h with the indicated concentrations of DS500 or HM2602. Infectious brain homogenate (0.25%) was added to the cells, which were further incubated for 18 h. (B) Duplicate wells were incubated for 24 h with Na chlorate (30 mM) or were left untreated (control). Infectious brain homogenate (0.25%) was added to the cells, which were further incubated for 18 h.