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IEM of membrane-enriched fraction. Fraction 9 of iodixanol gradients was processed for IEM using anti-E4 antibodies and goat anti-rabbit IgG labeled with 10-nm gold particles, as described in Materials and Methods. Images of different preparations of vesicles with particles associated are shown. Arrows indicate positive immunogold signals. Images of vesicles containing associated particles in the upper panels (white rectangles) are enlarged in the lower corresponding panels.
