FIG. 1.
Active immunization with both live avirulent EV71 strain and CA16 induced EV71 specific immunity in newborn mice. One-day-old ICR mice were orally inoculated with either live EV71/4643, CA16, or CB3 (104 PFU/mouse). (A) EV71 specific serum IgG and intestinal IgA levels were determined by ELISA at 7 and 14 days p.i. (B) Proliferation of splenocytes in response to EV71, CA16, or CB3 antigen stimulation was determined at 7 day p.i. The results are means ± the SEM (n = 10 to 12 mice). *, P < 0.05 compared to control mice.