FIG. 2.
Replication of M-tropic SHIVs in rhesus PBMC (A) or AM (B and C). Each SHIV preparation was spinoculated onto 5 × 106 cells of monkey PBMC (MOI, 5 × 10−3), and virus production was monitored by RT activity released into the medium (A). The replication of the parental T-cell-tropic SHIVDH12R-CL-7 is indicated by the thick line. AM (3 × 105) collected from the same donor animal on two different occasions were infected with the indicated viruses at an MOI of 3 × 10−2, and virus replication was assayed as described for PBMC (B and C).