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Serine 99 is essential for infectious virus production. (A) Replication of J6/JFH(p7-Rluc2A) genomes with serine-to-alanine substitutions in core at 8 h (light gray) and 48 h postelectroporation (dark gray). (B) Infectious virus production at 48 h postelectroporation. Means and SEM are shown for serine mutants; wild-type and polymerase-defective controls represent single electroporations.
