FIG. 9.

Summary of core functional sequences. (A) J6 core amino acid sequence. (B) Previously reported core functional regions (reviewed in reference 35). (C) Summary of results of alanine scanning mutagenesis of residues 57 to 191. Infectious virus production is indicated by shading: black, 50 to 100% J6/JFH(p7-Rluc2A); and gray, 0.3 to 1% wild-type. Mutation of residues highlighted by dashed boxes destabilized the core protein. Isolation of compensatory mutations indicated by stars. Residues mutated to alanine are numbered above.