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Effects of B-box 2 arginine changes on TRIMCyp. (A) The steady-state expression levels of the indicated TRIMCyp variants in Cf2Th cells were determined by Western blotting with an anti-HA antibody. wt, wild type. (B) Cf2Th cells expressing the indicated TRIMCyp variants were incubated with HIV-1-GFP. GFP-positive cells were counted. The results of a typical experiment are shown. The experiment was repeated with similar results.
