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Subcellular localization of TRIM5αrh and TRIM5αhu mutants. (A and B) Cf2Th cells stably expressing the indicated wild-type and mutant TRIM5α or TRIMCyp proteins were fixed and stained using a fluorescein isothiocyanate-conjugated anti-HA antibody as described in Materials and Methods. Representative confocal microscope images are shown.
