FIG. 3.
Alteration of two basic residues in the TRIM5αrh B-box 2 domain. Cells were transduced with either the empty LPCX vector or a vector expressing wild-type (wt) TRIM5αrh or the following TRIM5αrh mutants: K113A/R121A, C97A/H100A, K103A, the mutant with RING deleted [Δ(1-93)], the mutant with the RING-B box deleted [Δ(1-132)], or the mutant with the B-box deleted [Δ(97-129)]. (A) Cell lysates were analyzed by Western blotting using antibodies against HA (Anti-HA) and β-actin. (B to D) Cf2Th cells expressing the different TRIM5αrh variants were challenged with HIV-1-GFP (B), EIAV-GFP (C), or FIV-GFP (D). GFP-positive cells were counted. Similar results were obtained in three independent experiments.