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Effects of altering the charge on residues 113 and 121 of the TRIM5αrh B-box 2 domain. Cf2Th cells expressing the indicated wild-type and mutant TRIM5αrh proteins were challenged with HIV-1-GFP (A and D), EIAV-GFP (B), or FIV-GFP (C). GFP-positive cells were counted. Similar results were obtained in three independent experiments.
