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Effects of altering the charge on residues 111 and 119 of the TRIM5αhu B-box 2 domain. Cf2Th cells expressing the indicated wild-type and mutant TRIM5αhu proteins were challenged with N-MLV-GFP (A and B), EIAV-GFP (C), or FIV-GFP (D). GFP-positive cells were counted. Similar results were obtained in three independent experiments.
