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Oligomerization of TRIM5αrh and TRIM5αhu mutants. 293T cells were transiently transfected with plasmids expressing the indicated wild-type (wt) or mutant TRIM5α proteins with HA epitope tags. Cells expressing wild-type and mutant TRIM5αrh (A) or TRIM5αhu (B) proteins were lysed 48 h after transfection and cross-linked with the indicated concentrations of glycolbis-succinimidylsuccinate. The cell lysates were subsequently Western blotted with an anti-HA antibody. t, trimer; d, dimer.
