FIG. 8.

T98G cells that were positive for viral Ag continued to divide. (A) In order to investigate the possibility of Ag-positive-cell division, cells were arrested in metaphase by using a colchicine block. Cells were then stained for the indicated Ags. IE1⁺ cells were analyzed through P4, and an average of 15% of these IE1⁺ cells were found to be dividing during each passage. P2 d2, day 2 of P2. (B) Two examples of dividing cells, including phase images, are shown to emphasize the cytokinesis between daughter cells. In the top panels, cells were pulse-labeled with BrdU just prior to treatment with colchicine to visualize actively replicating viral DNA within replication centers. Equal division of viral genomes can be seen. P1 d1, day 1 of P1. Scale bars represent 5 μm.