TABLE 1.
Molecular profiling of natural and experimental ovine TSE strainsa
| Animal | Genotypeb | Breed (age in yr) | PrPres type within CM | PrPres withinc:
|
C2 withinc,d:
|
|||
|---|---|---|---|---|---|---|---|---|
| SC | Cer | CM | SC | Cer | ||||
| 0455e | VRQ/VRQ | Swaledale (6) | Scrapie | + | + | + | + | − |
| 0226e | ARQ/VRQ | Swaledale (5) | Scrapie | + | + | + | + | − |
| 0456e | ARQ/VRQ | Swaledale (3) | Scrapie | + | − | + | + | − |
| 0284f | AHQ/AHQ | Finn Dorset (unknown) | Scrapie | + | − | − | − | − |
| 0615e | ARQ/VRQ | Swaledale (5) | Scrapie | + | + | + | + | + |
| 0923g | VRQ/VRQ | Welsh Hill Speckled (2) | Scrapie | + | + | + | + | + |
| 0925g | VRQ/VRQ | Welsh Hill Speckled (5) | Scrapie | + | + | + | + | + |
| 0836e | ARQ/VRQ | Mule (2) | Scrapie | + | + | + | + | + |
| 1276g | VRQ/VRQ | Welsh Mountain (2) | Scrapie | + | + | + | + | + |
| 1563g | VRQ/VRQ | Bleu De Maine (3) | Scrapie | + | + | + | + | + |
| 1275g | VRQ/VRQ | Welsh Mountain (2) | Scrapie | + | + | + | + | + |
| 0210e | ARQ/ARQ | Warborough (2) | Scrapie | ND | + | ND | ND | ND |
| 0635e | ARQ/ARQ | Charollais Cross (3) | Scrapie | ND | + | ND | ND | ND |
| 0678e | ARQ/ARQ | Suffolk Cross (3) | Scrapie | ND | + | ND | ND | ND |
| 0575e | ARQ/ARQ | Cambridge (3) | Scrapie | ND | ND | ND | ND | ND |
| 0392g,h | ARQ/ARQ | Romney (3) | BSE | ND | + | − | ND | − |
| 1693g,h | ARQ/ARQ | Romney (2) | BSE | ND | + | − | ND | − |
| 0654g,h | ARQ/ARQ | Romney (3) | BSE | ND | + | − | ND | − |
| 1842g,h | ARQ/ARQ | Romney (unknown) | BSE | ND | ND | ND | ND | ND |
| CH1641g,h | AHQ/AHQ | Cheviot (1) | ND | ND | − | ND | ND | − |
| SSBP1g,h | VRQ/VRQ | Cheviot (2) | ND | ND | + | ND | ND | + |
Shown are thermolysin PrPres profiles and results for the detection of truncated PrP C2 species. +, PrPres or C2 fragment was detected; −, PrPres or C2 fragment was not detected; ND, not determined.
Amino acid residues at positions 136, 154, and 171.
Thermolysin-resistant PrP or C2 when present within spinal cord (SC) and cerebellum (Cer) samples gave BSE- or scrapie-specific molecular weight profiles as indicated for the caudal medulla (CM) samples.
A total of 3.3 μl of 2% (wt/vol) brain homogenate was analyzed. All samples were analyzed at least in triplicate.
Tissues were frozen within 1 h of death.
For animal 0284, how long after death tissues were snap-frozen is not known; however, no autolysis was noted for the tissue.
Tissues were frozen within 12 h of death.
Experimentally infected animals (all other animals are natural infections).