FIG. 6.

Fluorescence recovery after photobleaching (FRAP) analysis of trafficking of the gM/gN, gMΔAC/gN, and gMΔYQAL/gN protein complexes in the ACs of virus-infected cells. Plasmids encoding GFP-tagged wild-type gM (gMwt), gMΔAC, and gMΔYQAL were electroporated into HFs and plated on 13-mm coverslips, and 24 h later, these cells were infected with the respective viruses. Once a well-formed AC was established, the live cells were subjected to FRAP as described in Materials and Methods. (A) Fluorescence recovery following photobleaching of the AC to 20% of initial fluorescence intensity as a function of time (300 s). Note the continued fluorescence recovery of wild-type gM/gN at 300 s compared to the plateau in recovery reached by both gMΔAC/gN and gMΔYQAL/gN at that time point. The error bars indicate standard errors. (B) Examples of the images collected during FRAP analysis of the AC and following fluorescence recovery.