FIG. 3.
PRV can spread interaxonally in a gD-independent manner from neuron to neuron in the absence of target cells in the M compartment. (A) SCG neurons were plated in both S and N compartments. At 6 days after plating, neurons in the S compartment were infected with PRV 151 and PRV GS442 at a high MOI. After 24 h, the total contents of the S and N compartments were harvested and titers were determined for PK15 cells. Five chambers were used for each set of infections. The open circle beside each data set represents the average value for that particular set of data. The standard deviations are ±1.5 × 105 for the S compartment and ±3.9 × 103 for the N compartment. (B) Chambers were set up and infected with PRV as described above. At 24 h postinfection, the EGFP expressed in cells infected with PRV 151 (a to d) or PRV GS442 (e to h) was imaged live using an epifluorescence microscope (b, d, f, and h).