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. 2007 Jul 25;81(19):10486–10495. doi: 10.1128/JVI.00808-07

FIG. 1.

FIG. 1.

Ad-BCL-xL recombinants and protein expression. (A) Map of the E1B region of Ad-BCL-xL recombinant. The portion between nucleotide position 1710 (EcoNI) to 3328 (BglII) in ΔE1B is deleted. The deleted region is substituted with wt or mutant BCL-xL coding sequences under the transcriptional control of the CMV immediate-early promoter. (B) Expression of BCL-xL and mutant proteins. A549 cells were infected with the indicated viruses, and the expression of BCL-xL protein was analyzed by Western blotting. (C) Interaction of BCL-xL and E1B-19K with BAK. The whole-cell lysates (WCL) were either immunoprecipitated (IP) with the 19K antibody or BCL-xL antibody, and the blots were probed with the BAK antibody (Upstate, NY).