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. Author manuscript; available in PMC: 2008 Sep 1.
Published in final edited form as: J Mol Cell Cardiol. 2007 Jun 6;43(3):281–291. doi: 10.1016/j.yjmcc.2007.05.022

Fig. 3.

Fig. 3

Activation of PKA is required for the CaMKII-dependent phosphorylation of Ser2815 of RyR2 and Thr17 of PLN. Representative immunoblots and overall results of SR vesicles isolated from hearts perfused without (Ctrl) and with 30 nM isoproterenol in the absence (Iso) and the presence of 30 μM of the PKA inhibitor H-89 (Iso-H-89) and with 1 μM of forskolin (FK). Phosphorylation of Ser2815 of RyR2 and Thr17 of PLN is shown as ratio between the signals of the site-specific phospho-antibody and the antibody used to recognized the non-phosphorylated form. *p<0.05 with respect to Ctrl. #p<0.05 with respect to Iso-treated hearts.