Table 4.
Candidate Alu loci amenable to PCR and PCR amplification conditions.
| Locus
name |
Genomic
location |
Forward primer (5’>3’) | Reverse primer (5’>3’) | Annealing
temperature |
PCR product size
(filled/empty site) |
|---|---|---|---|---|---|
| RC2 | 8p11.21 | TCATCTTGACCTTGCAGCAC | CCGTAACAGGACAGCTCACA | 60 | 420/120 |
| RC3 | 3p24.1 | TGCCCAAATGTAGACCTTGT | TTGTTGGAGTTGAGGCATCTT | 59 | 460/160 |
| RC5 | 7q31.31 | CCCCTTCCAGAGAAGCATTT | GCTCTTCTTTTTCAGTGAGTTTCC | 60 | 460/160 |
| RC6 | 12q23.3 | CATATGCACCGCGCTAAGTA | TCCTAATGCCTTTTTCCATAACA | 60 | 470/170 |
| A1 | 14q31.3 | TTGAAAAGGGGGTAGTTTATGA | AACCCTTGAGAGGGGATGAC | 55 | 710/384 |
| E1 | 15q25.2 | GCCCTCTAGGAAAGTGAAAGAA | GCCATAGGTTTCCTCTGGTTG | 55 | 570/242 |
| E2 | 13q12.3 | CCAAGACCCAGGCATTAAAA | GAAGGATGGTGATTGCAGGT | 55 | 523/202 |
| E4 | 5q14.3 | TATGCATAGCCAAAAGAGAGCA | ACATTTGGGACATCAGGGTAAC | 60 | 497/197 |