TABLE 1.

FMDV passage in vivo and in vitro

Pig no.	Pig passage	Avg TCID50 (n = 4)a	Observations	BHK-21 cell passage	Avg TCID50 (n = 4)a
48	T00	5.92 ± 0.54	Injected to produce stock	P1A	5.8 ± 0.51
50	T00	5.81 ± 1.1	Injected to produce stock	P1B	5.8 ± 0.39
483	T0	7.30 ± 0	Injected, in contact with two naive pigsb	P2A	7.6 ± 0.54
484	T1	7.13 ± 0.67		P2B	6.3 ± 0.51
485	T1	7.97 ± 0.17		P3A	6.8 ± 1.55
486	T2	5.79 ± 0.3		P3B	5.8 ± 1.2
487	T2	7.58 ± 0.39		P4A	6.6 ± 0.51
488	T3	5.29 ± 0.86		P4B	6.6 ± 0.72
489	T3	3.5 ± 0.9		P5A	6.5 ± 0.5
490	T4	7.09 ± 0.46		P5B	6.5 ± 0.22
491	T4	8.34 ± 1.04		P6A	6.8 ± 0.72
492	T5	5.8 ± 1.0		P6B	6.6 ± 0.5
493	T5	5.93 ± 0.37		P7A	6.1 ± 0.05
494	T6	7.75 ± 0.05		P7B	6.8 ± 0.37
495	T6	6.46 ± 0.16		P8A	6.3 ± 0.54
496	T7	5.3 ± 0.83		P8B	7.3 ± 1.55
497	T7	7.8 ± 0.63		P9A	6.3 ± 0.27
498	T8	6.74 ± 1.4		P9B	7.3 ± 1.04
499	T8	5.35 ± 0.78		P10A	6.6 ± 0.3
725	T9	3.07 ± 0.27		P10B	6.6 ± 0.39
726	T9	7.07 ± 0.44		P11A	6.8 ± 0.51
4500	T10	5.21 ± 3.9		P11B	6.8 ± 0.27
5008	T10	1.84 ± 1		P12A	7.5 ± 0.05
5009	T11	4.02 ± 0.45		P12B	6.1 ± 0.17
5010	T11	6.85 ± 0.27		P13A	6.5 ± 0.54
5011	T12	5.57 ± 0.22		P13B	6.8 ± 1.04
5012	T12	6.41 ± 0.89		P14A	7.1 ± 0.72
5013	T13	8.3 ± 0.17		P14B	6.0 ± 0.39
5014	T13	5.9 ± 0.61		P15A	7.0 ± 0.45
5015	T14	6.21 ± 0.6	Contact transmitted with no clinical signs	P15B	7.0 ± 0.27
5016	T14	<0.8	Contact transmitted with no clinical signs	P16A	7.0 ± 0.54
4823	T15	5.85 ± 0.5	Injected with 5015 vesicular fluidc	P16B	6.8 ± 0.61
4824	T16	5.75 ± 0.72	In contact with 4823	P17A	7.3 ± 0.16
4825	T17	6.92 ± 0.51		P17B	6.6 ± 0.51
4826	T17	<0.8		P18A	7.0 ± 0.89
5184	T18	<0.8	Contact transmitted with no clinical signs	P18B	6.8 ± 0.44
5185	T18	1.97 ± 1.2	Contact transmitted with no clinical signs	P19A	7.3 ± 1.04
5005	T19	3.42 ± 1.55	Injected with 5185 vesicular fluidd	P19B	6.6 ± 0.22
5006	T20	<0.8	Contact transmitted with no clinical signs	P20A	7.1 ± 0.17
5007	T20	<0.8	Contact transmitted with no clinical signs	P20B	6.8 ± 0.05

^aTiters are expressed as log₁₀ numbers of of TCID₅₀/ml. Titer values represent the means and standard deviations for four different vesicles from the same pig or four replicates of titration of supernatant from the same cell culture.

^bPig 483 was inoculated i.d. with 10^6.37 TCID₅₀ from original viral stock T00.

^cPig 4823 was inoculated i.d. with 10^5.7 TCID₅₀ from pig 5015 vesicular fluid.

^dPig 5005 was inoculated with 10^1.15 TCID₅₀ from pig 5185 vesicular fluid.