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. 2007 Aug 1;81(20):10869–10878. doi: 10.1128/JVI.00542-07

FIG. 4.

FIG. 4.

Comparison of chimeric HIV-1 Env incorporation into VLPs. (A) Schematic diagram of additional chimeric HIV-1 Con-S Env constructs. B-TM.CTBV, Con-S ΔCFI with SP and TM-CT domains derived from BV gp64; C-TM.CTHA, Con-S ΔCFI with chitinase SP and influenza virus HA TM-CT domains. (B) In the upper left panel, 1 μg of M-TM.CTMMTV, C-TM.CTHA, B-TM.CTBV, Con-S gp160, or HIV-1 Gag VLPs was analyzed by Western blotting. In the upper right panel, 10 μg of Con-S gp160 VLPs was loaded for the Western blot; in the lower panel, Env and Gag contents were quantitated by sandwich ELISA. *, μg/100 μg of the VLP protein. †, The molecular masses used for the ratio calculation were as follows: M-TM.CTMMTV, C-TM.CTHA, and B-TM.CTBV, 145 kDa; Con-S gp160, 160 kDa; and Gag, 55 kDa.