FIG. 5.

Western blot analysis of VLPs produced using LFV GP-derived CT or TM-CT chimeric Env proteins. (A) Schematic diagram of chimeric Con-S Env fused with LFV GP-derived CT/TM-CT. The coding sequence for the LFV glycoprotein CT (LFV GP aa 450 to 491) or TM-CT (LFV GP aa 427 to 491) was fused to that of the C-terminal end of Con-S ΔCFI. (B) Western blot of protein expression in cell lysates and VLPs probed using goat anti-HIV-1 gp120 antibody. (C) Western blot of VLP matrix proteins (1 μg/well) released in VLPs probed with a mixture of mouse anti-HIV-1 Gag and anti-Lassa Z antibody mixture. (D) LFV Z VLPs, which incorporated the LFV GP. LFV VLPs were harvested from the culture supernatants of insect cells infected with rBVs expressing LFV GP and Z and resolved by SDS-PAGE, and the blot was probed with antibodies specific to LFV GP2 and Z. Each lane contains 5 μg of VLPs.