TABLE 1.
Quantitation of HIV pseudotype assembly and infectivity
| Pseudotype | Assemblya (108 GE/ml) | Infectivityb (104 TU/ml)
|
|||
|---|---|---|---|---|---|
| PHH | PWH | Huh7 | 293T | ||
| HIV(G) | 1.4 | 4.0 | 2.8 | 48 | 440 |
| HIV(LMS) | 7.0 | 10 | <0.0005 | <0.0005 | <0.0005 |
| HIV(S) | 5.6 | <0.0005 | <0.0005 | <0.0005 | <0.0005 |
a
Assembly was quantitated by qPCR for the LacZ reporter sequence to determine the HIV GE/ml.
b
Infectivity was determined from assays at 3 days after infection of β-galactosidase-positive cells, where each positive cell is counted as 1 TU. The titer of the inoculum in TU/ml was deduced as the average result of infections performed in duplicate.