FIG. 3.

rPMP is incorporated into genomic RNA during transcription with T7 RNAP. (A) HPLC separation of RNA transcribed in the presence of rPTP. RNA was digested to component nucleosides as described in the text. rP was observed along with the four natural nucleosides. (B) rPMP composition of RNA (as determined by HPLC of digested RNA) is plotted against the concentration of rPTP present in the in vitro transcription reaction mixture. The PV genome is 7.5 kb in length. (C) RNA transcribed in the presence of increasing concentrations of rPTP was run on a 1% agarose gel and stained with ethidium bromide. Differences in RNA quality or length were not observed when rPTP was added to the transcription reaction mixture at up to 20% of the total nucleotide pool. L, DNA ladder.